Structure and expression of mouse aldolase genes. Brain-specific aldolase C amino acid sequence is closely related to aldolase A
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چکیده
منابع مشابه
Amino acid sequence of a fragment of rabbit muscle aldolase.
Cleavage of rabbit muscle aldolase by cyanogen bromide results in the formation of four fragments of different size [1]. Study of the primary structure of the enzyme has been based on the examination of these fragments [1, 2]. Because of the insolubility and associated problems due to the relatively large size of the fragments Lai [1], as well as Anderson et al. [3], used pyridine-acetic acid b...
متن کاملThe carboxyl-terminal structure of rabbit liver aldolase (aldolase B).
The amino acid residues in the carboxyl-terminal region of fructose diphosphate aldolase isolated from fresh rabbit liver are -(Gly,Phe,Leu,Ala,Thr2,Ser2)-Tyr. This differs from the carboxyl-terminal structure in aldolase isolated from tissue which had been frozen and thawed. Amino acid analyses, ultracentrifugation, and digestion with carboxypeptidase suggest that the difference is the result ...
متن کامل[Aldolase].
OH OH Two main classes of aldolases are found in Nature. Type I aldolases activate the nucleophilic partner by forming an imine as an intermediate; this type of enzyme is found in animals and higher plants. Type II aldolases occur in microorganisms and they require R 2 to be a hydroxy group. These enzymes feature a Zn 2+ ion that promotes the reaction by forming a chelate with both the carbonyl...
متن کاملComplete amino acid sequence for human aldolase B derived from cDNA and genomic clones.
Several aldolase B clones from a human liver cDNA library have been identified by using a rabbit aldolase A cDNA as a hybridization probe. The most complete of these, pHL413, is 1389 base pairs long and covers approximately equal to 80% of the length of the mRNA, including 90% of the translated region. The cDNA, pHL413, was used to identify a genomic clone, lambda HG313, which encoded the remai...
متن کاملThe complete amino acid sequence of human skeletal-muscle fructose-bisphosphate aldolase.
The complete amino acid sequence of human skeletal-muscle fructose-bisphosphate aldolase, comprising 363 residues, was determined. The sequence was deduced by automated sequencing of CNBr-cleavage, o-iodosobenzoic acid-cleavage, trypsin-digest and staphylococcal-proteinase-digest fragments. Comparison of the sequence with other class I aldolase sequences shows that the mammalian muscle isoenzym...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1986
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1986.tb09572.x